| Téléchargement | - Voir la version finale : Simplifying glycan monitoring of complex antigens such as the SARS-CoV-2 spike to accelerate vaccine development (PDF, 2.1 Mio)
- Voir les données supplémentaires : Simplifying glycan monitoring of complex antigens such as the SARS-CoV-2 spike to accelerate vaccine development (ZIP, 5.2 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1038/s42004-023-00988-1 |
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| Auteur | Rechercher : Sauvageau, Janelle1Identifiant ORCID : https://orcid.org/0000-0001-7476-8424; Rechercher : Koyuturk, Izel1; Rechercher : St. Michael, Frank1; Rechercher : Brochu, Denis1; Rechercher : Goneau, Marie-France1; Rechercher : Schoenhofen, Ian1; Rechercher : Perret, Sylvie1; Rechercher : Star, Alexandra1Identifiant ORCID : https://orcid.org/0000-0001-9524-4312; Rechercher : Robotham, Anna1Identifiant ORCID : https://orcid.org/0000-0002-6464-736X; Rechercher : Haqqani, Arsalan1; Rechercher : Kelly, John1Identifiant ORCID : https://orcid.org/0000-0003-1998-3572; Rechercher : Gilbert, Michel1Identifiant ORCID : https://orcid.org/0000-0002-0204-4408; Rechercher : Durocher, Yves1Identifiant ORCID : https://orcid.org/0000-0002-2268-4111 |
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| Affiliation | - Conseil national de recherches Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Sujet | Coronavirinae; SARS Coronavirus; COVID-19 |
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| Résumé | Glycosylation is a key quality attribute that must be closely monitored for protein therapeutics. Established assays such as HILIC-Fld of released glycans and LC-MS of glycopeptides work well for glycoproteins with a few glycosylation sites but are less amenable for those with multiple glycosylation sites, resulting in complex datasets that are time consuming to generate and difficult to analyze. As part of efforts to improve preparedness for future pandemics, researchers are currently assessing where time can be saved in the vaccine development and production process. In this context, we evaluated if neutral and acidic monosaccharides analysis via HPAEC-PAD could be used as a rapid and robust alternative to LC-MS and HILIC-Fld for monitoring glycosylation between protein production batches. Using glycoengineered spike proteins we show that the HPAEC-PAD monosaccharide assays could quickly and reproducibly detect both major and minor glycosylation differences between batches. Moreover, the monosaccharide results aligned well with those obtained by HILIC-Fld and LC-MS. |
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| Date de publication | 2023-09-08 |
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| Maison d’édition | Springer Nature |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | 988 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | cb398606-01fe-43e3-85b6-af070bde9afe |
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| Enregistrement créé | 2024-07-30 |
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| Enregistrement modifié | 2024-07-30 |
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