Digital in-line holography: 4-D imaging and tracking of micro-structures and organisms in microfluidics and biology

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1117/12.667447
Auteur	Rechercher : Garcia-Sucerquia, J.; Rechercher : Xu, W.; Rechercher : Jericho, S. K.; Rechercher : Jericho, M. H.; Rechercher : Tamblyn, I.; Rechercher : Kreuzer, H. J.
Éditeur	Rechercher : Von Bally, Gert; Rechercher : Luo, Qingming
Format	Texte, Article
Conférence	ICO20: Biomedical Optics, 21 August 2005, Changchun, China
Résumé	In recent years, in-line holography as originally proposed by Gabor, supplemented with numerical reconstruction, has been perfected to the point at which wavelength resolution both laterally and in depth is routinely achieved with light by using digital in-line holographic microscopy (DIHM). The advantages of DIHM are: (1) simplicity of the hardware (laser- pinhole-CCD camera), (2) magnification is obtained in the numerical reconstruction, (3) maximum information of the 3-D structure with a depth of field of millimeters, (4) changes in the specimen and the simultaneous motion of many species, can be followed in 4-D at the camera frame rate. We present results obtained with DIHM in biological and microfluidic applications. By taking advantage of the large depth of field and the plane-to-plane reconstruction capability of DIHM, we can produce 3D representations of the paths followed by micron-sized objects such as suspensions of microspheres and biological samples (cells, algae, protozoa, bacteria). Examples from biology include a study of the motion of bacteria in a diatom and the track of algae and paramecium. In microfluidic applications we observe micro-channel flow, motion of bubbles in water and evolution in electrolysis. The paper finishes with new results from an underwater version of DIHM.
Date de publication	2006-02-02
Maison d’édition	Society of Photo-Optical Instrumentation Engineers
Dans	ICO20: Biomedical Optics, 602613.
Série	Proceedings of SPIE.
Langue	anglais
Publications évaluées par des pairs	Oui
Publication du CNRC	Cette publication n’est pas du CNRC Ce sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC.
Numéro NPARC	23003175
Exporter la notice	Exporter en format RIS
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Identificateur de l’enregistrement	cbb880df-5e22-423e-aa86-fc494d62feb5
Enregistrement créé	2018-05-03
Enregistrement modifié	2020-04-22

Date de modification :: 2024-07-04