| DOI | Trouver le DOI : https://doi.org/10.1016/j.lfs.2003.10.021 |
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| Auteur | Rechercher : Banskota, Arjun H.; Rechercher : Nguyen, Nhan Trung; Rechercher : Tezuka, Yasuhiro; Rechercher : Le Tran, Quan; Rechercher : Nobukawa, Takahiro; Rechercher : Kurashige, Youichi; Rechercher : Sasahara, Masakiyo; Rechercher : Kadota, Shigetoshi |
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| Format | Texte, Article |
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| Sujet | Secoisolariciresinol; Isotaxiresinol; Apoptosis; Taxus yunnanensis; Hepatoprotective effect; TNF-α; D-Galactosamine; Lipopolysaccharide; Lignan |
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| Résumé | effects of secoisolariciresinol (1) and isotaxiresinol (2), two major lignans isolated from the wood of Taxus yunnanensis, on tumor necrosis factor-α (TNF-α)-dependent hepatic apoptosis induced by D-galactosamine (d-GalN)/lipopolysaccharide (LPS) were investigated in mice. Co-administration of d-GalN (700 mg/kg) and LPS (10 μg/kg) resulted in a typical hepatic apoptosis characterized by DNA fragmentation and the formation of apoptotic bodies. Serum glutamic pyruvic transaminase (sGPT) and glutamic oxaloacetic transaminase (sGOT) levels were also raised at 8 h after d-GalN/LPS intoxication due to a severe necrosis of hepatocytes. Pre-administration of 1 or 2 (50, 10 mg/kg, i.p.) 12 and 1 h before d-GalN/LPS significantly reduced DNA fragmentation and prevented chromatin condensation, apoptotic body formation and hepatitis. Pro-inflammatory cytokines such as TNF-α and interferon-γ (IFN-γ) secreted from LPS-activated macrophages are important mediators of hepatocyte apoptosis in this model. Pre-treatment with 1 or 2 significantly inhibited the elevation of serum TNF-α and IFN-γ levels. In a separate experiment, both lignans had a significant dose-dependent protective effect on d-GalN/TNF-α-induced cell death in primary cultured mouse hepatocytes and TNF-α-mediated cell death in murine L929 fibrosarcoma cells. These results indicated that 1 and 2 prevent d-GalN/LPS-induced hepatic injury by inhibiting hepatocyte apoptosis through the blocking of TNF-α and IFN-γ production by activated macrophages and direct inhibition of the apoptosis induced by TNF-α. |
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| Date de publication | 2004-03-18 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Publication du CNRC | Cette publication n’est pas du CNRCCe sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC. |
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| Numéro NPARC | 21274279 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | cbdce5f1-6757-45f0-8885-e14d44d6e7df |
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| Enregistrement créé | 2015-03-09 |
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| Enregistrement modifié | 2020-04-17 |
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