¹H-NMR and mass spectrometric characterization of the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1007/s11306-008-0144-0
Auteur	Rechercher : Karakach, Tobias K.¹; Rechercher : Huenupi, Elizabeth C.¹; Rechercher : Soo, Evelyn C.¹; Rechercher : Walter, John A.¹; Rechercher : Afonso, Luis O. B.¹
Affiliation	Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
Format	Texte, Article
Résumé	Stressors of various kinds constantly affect fish both in the wild and in culture, examples being acute water temperature and quality changes, predation, handling, and confinement. Known physiological responses of fish to stress such as increases in plasma cortisol and glucose levels, are considered to be adaptive, allowing the animal to cope in the short term. Prolonged exposure to stressors however, has the potential to affect growth, immune function, and survival. Nonetheless, little is known about the mechanisms underlying the long-term stress response. We have investigated the metabolic response of juvenile Atlantic salmon (Salmo salar) to long-term handling stress by analyzing fish plasma via 1H nuclear magnetic resonance spectroscopy and ultra high performance liquid chromatography–mass spectrometry (UPLC–MS), and comparing results with controls. Analysis of NMR data indicated a difference in the metabolic profiles of control and stressed fish after 1 week of stress with a maximum difference observed after 2 weeks. These differences were associated with stress-induced increases in phosphatidyl choline, lactate, carbohydrates, alanine, valine and trimethylamine- N-oxide, and decreases in low density lipoprotein, very low density lipoprotein, and lipid. UPLCMS data showed differences at week 2, associated with another set of compounds, tentatively identified on the basis of their mass/charge. Overall the results provided a multi-faceted view of the response of fish to long-term handling stress, indicating that the metabolic disparity between the control and stress groups increased to week 2, but declined by weeks 3 and 4, and revealed several new molecular indicators of long-term stress.
Date de publication	2009
Dans	Metabolomics 5, nº 1 (2009) : 123–137.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	NRCC 42805 1791
Numéro NPARC	3538299
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	cc01d34f-e06d-4876-b34b-1c1f6c2c1c2f
Enregistrement créé	2009-03-01
Enregistrement modifié	2020-04-16

Date de modification :: 2024-06-26