QTL mapping and molecular characterization of the classical D locus controlling seed and flower color in Linum usitatissimum (flax)

Par Conseil national de recherches du Canada

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DOI	Trouver le DOI : https://doi.org/10.1038/s41598-017-11565-7
Auteur	Rechercher : Sudarshan, Gurudatt Pavagada; Rechercher : Kulkarni, Manoj¹; Rechercher : Akhov, Leonid¹; Rechercher : Ashe, Paula¹; Rechercher : Shaterian, Hamid¹; Rechercher : Cloutier, Sylvie; Rechercher : Rowland, Gordon¹; Rechercher : Wei, Yangdou; Rechercher : Selvaraj, Gopalan¹
Affiliation	Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
Format	Texte, Article
Résumé	The flowers of flax (linseed) are blue-hued, ephemeral and self-pollinating, and the seeds are typically brown. A century-old interest in natural yellow seed variants and a historical model point to recessive alleles in B1, D and G loci being responsible, but the functional aspects had remained unknown. Here, we characterized the “D” locus by quantitative trait loci (QTL) mapping and identified a FLAVONOID 3′5′ HYDROXYLASE (F3′5′H) gene therein. It does not belong to the F3′5′H clade, but resembles biochemically characterized F3′Hs (flavonoid 3′ hydroxylase) but without F3′H activity. The genome lacks other F3′H or F3′H-like genes. The apparent neo-functionalization from F3′H is associated with a Thr498 → Ser498 substitution in a substrate recognition site (SRS). The yellow seed and white flower phenotypes of the classical d mutation was found to be due to one nucleotide deletion that would truncate the deduced product and remove three of the six potential SRS, negatively impacting delphinidin synthesis. Delphinidin is sporadic in angiosperms, and flax has no known pollination syndrome(s) with functional pollinator group(s) that are attracted to blue flowers, raising questions on the acquisition of F3′5′H. The appearance of d allele is suggestive of the beginning of the loss of F3′5′H in this species.
Date de publication	2017-11-16
Maison d’édition	Nature Publishing Group
Dans	Scientific Reports, nº 7, 15751.
Note	Erratum published online 12 March 2018 DOI: 10.1038/s41598-018-22651-9
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	NRC-ACRD-56361 NRC-ACRD-PDB011
Numéro NPARC	23002777
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	d5dbe07c-fce3-4a79-a1d5-bc23f59666f9
Enregistrement créé	2018-02-05
Enregistrement modifié	2020-05-30

Date de modification :: 2024-09-18