DOI | Trouver le DOI : https://doi.org/10.1002/elan.200403117 |
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Auteur | Rechercher : Luong, John H. T.1; Rechercher : Hrapovic, Sabahudin1; Rechercher : Wang, Dashan2 |
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Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
- Conseil national de recherches du Canada. Institut de technologie des procédés chimiques et de l'environnement du CNRC
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Format | Texte, Article |
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Sujet | environmental |
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Résumé | Y-Aminopropyltriethoxysilane (APTES)-induced solubilization of multi-wall carbon nanotube CNTs allowed for the modification of electrode surfaces. APTES also served as an immobilization matrix for putrescine oxidase (POx) to construct an amperometric biosensor. Although CNTs modified by APTES acted as semiconductors to reduce the exposed sensing surface, we reasoned that nanoscale dendrites of CNTS modified by APTES formed a network and projected outwards from the electrode surface and acted like bundled ultra-microelectrodes that allowed access to the active site and facilitated direct electron transfer to the immobilized enzyme. Our biosensor was able to efficiently monitor direct electroactivity of POx at the electrode surface. The putrescine biosensor prepared using the modified glassy carbon electrode exhibited current response within 10 s with a detection limit of 500 nM. |
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Date de publication | 2005 |
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Dans | |
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Numéro du CNRC | 47209 |
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Numéro NPARC | 3540331 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | d6273142-7621-4f4c-85b2-05ca594595d2 |
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Enregistrement créé | 2009-03-01 |
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Enregistrement modifié | 2020-04-07 |
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