| Auteur | Rechercher : Steiner, K.; Rechercher : Novotny, R.; Rechercher : Patel, K.; Rechercher : Vinogradov, Evguenii; Rechercher : Whitfield, C.; Rechercher : Valvano, M.; Rechercher : Messner, P.; Rechercher : Schaffer, C. |
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| Format | Texte, Article |
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| Sujet | Amino Acid Sequence; Bacillaceae; Bacteria; bacterial; Bacterial Proteins; biosynthesis; Carbohydrate Sequence; CHAIN; chemistry; CLUSTER; Conserved Sequence; ENZYME; Escherichia; Escherichia coli; ESCHERICHIA-COLI; Galactosyltransferases; GENE; genetics; GLYCAN CHAIN; glycoprotein; Glycoproteins; Glycosylation; In Vitro; Linker; Lipopolysaccharides; membrane; Membrane Glycoproteins; metabolism; Molecular Sequence Data; Plasmids; POLYSACCHARIDE; Polysaccharides; protein; Protein Conformation; REPEATING UNIT; RESIDUES; Salmonella; Sequence Alignment; Sequence Homology,Amino Acid; STRAIN; STRAINS; surface; transfer; transferase; TRISACCHARIDE; UNIT |
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| Résumé | The glycan chain of the S-layer glycoprotein of Geobacillus stearothermophilus NRS 2004/3a is composed of repeating units [-->2)-alpha-l-Rhap-(1-->3)-beta-l-Rhap-(1-->2)-alpha-l-Rhap-(1-->], with a 2-O-methyl modification of the terminal trisaccharide at the nonreducing end of the glycan chain, a core saccharide composed of two or three alpha-l-rhamnose residues, and a beta-d-galactose residue as a linker to the S-layer protein. In this study, we report the biochemical characterization of WsaP of the S-layer glycosylation gene cluster as a UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase that primes the S-layer glycoprotein glycan biosynthesis of Geobacillus stearothermophilus NRS 2004/3a. Our results demonstrate that the enzyme transfers in vitro a galactose-1-phosphate from UDP-galactose to endogenous phosphoryl-polyprenol and that the C-terminal half of WsaP carries the galactosyltransferase function, as already observed for the UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase WbaP from Salmonella enterica. To confirm the function of the enzyme, we show that WsaP is capable of reconstituting polysaccharide biosynthesis in WbaP-deficient strains of Escherichia coli and Salmonella enterica serovar Typhimurium |
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| Date de publication | 2007-04 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | STEINER2007 |
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| Numéro NPARC | 9364325 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | d6804f6f-3573-4485-b800-2872911318b9 |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2022-11-18 |
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