Téléchargement | - Voir la version finale : A novel human induced pluripotent stem cell blood-brain barrier model: applicability to study antibody-triggered receptor-mediated transcytosis (PDF, 2.2 Mio)
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DOI | Trouver le DOI : https://doi.org/10.1038/s41598-018-19522-8 |
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Auteur | Rechercher : Ribecco-Lutkiewicz, Maria1; Rechercher : Sodja, Caroline1; Rechercher : Haukenfrers, Julie1; Rechercher : Haqqani, Arsalan S.1; Rechercher : Ly, Dao1; Rechercher : Zachar, Peter1; Rechercher : Baumann, Ewa1; Rechercher : Ball, Marguerite1; Rechercher : Huang, Jez; Rechercher : Rukhlova, Marina1; Rechercher : Martina, Marzia1; Rechercher : Liu, Qing; Rechercher : Stanimirovic, Danica1; Rechercher : Jezierski, Anna1; Rechercher : Bani-Yaghoub, Mahmud |
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Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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Format | Texte, Article |
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Résumé | We have developed a renewable, scalable and transgene free human blood-brain barrier model, composed of brain endothelial cells (BECs), generated from human amniotic fluid derived induced pluripotent stem cells (AF-iPSC), which can also give rise to syngeneic neural cells of the neurovascular unit. These AF-iPSC-derived BECs (i-BEC) exhibited high transendothelial electrical resistance (up to 1500 Ω cm2) inducible by astrocyte-derived molecular cues and retinoic acid treatment, polarized expression of functional efflux transporters and receptor mediated transcytosis triggered by antibodies against specific receptors. In vitro human BBB models enable pre-clinical screening of central nervous system (CNS)-targeting drugs and are of particular importance for assessing species-specific/selective transport mechanisms. This i-BEC human BBB model discriminates species-selective antibody- mediated transcytosis mechanisms, is predictive of in vivo CNS exposure of rodent cross-reactive antibodies and can be implemented into pre-clinical CNS drug discovery and development processes. |
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Date de publication | 2018-01-30 |
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Maison d’édition | Nature Publishing Group |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 23002774 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | d891f0d8-7b5e-4fc6-b321-22e16d6b338a |
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Enregistrement créé | 2018-02-05 |
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Enregistrement modifié | 2020-05-30 |
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