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Jun and JNK kinase are activated in thymocytes in response to VM26 and radiation but not glucocorticoids

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1006/excr.1996.3419
AuteurRechercher : ; Rechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
FormatTexte, Article
Résumé
In order to establish what role members of the activating protein-1 (AP-1) gene families, i.e., c-fos,c-jun, junB,andjunD,play in thymic apoptosis, we have analyzed changes in their expression in response to three different agents: a glucocorticoid analog dexamethasone, an inhibitor of topoisomerase II teniposide VM26, and gamma radiation. All three agents induced thymic death at a similar rate and with the same morphological and biochemical features. There was a rapid and transient increase in the steady-state mRNA level ofjunBand c-fosgenes in all treatments, including control cultures, reminiscent rather of cellular stress response to the environmental changes than to the apoptotic stimuli. On the other hand, treatments with the DNA-damaging agents, VM26 and gamma radiation, resulted in superinduction of the c-junmRNA and in the activation of the stress response signaling pathway of c-Jun N-terminal kinase. Gene transcription ceased completely in cells with fragmented DNA and the down-regulation of genes such asjunDandtubulinwas reflective of the thymocytes’ commitment to apoptosis. The DNA-binding activities of the serum response factors, cyclic AMP response element binding proteins, and AP-1 factors, indicative of their transcriptional competence, were compromised shortly after induction of apoptosis regardless of the agent employed, consistent with previously reported enhancement in cellular proteolysis which is an essential component of the apoptotic cell death.
Date de publication
Dans
  • Experimental Cell Research 230, nº 2 (1 février 1997) : 220–232.
Langueanglais
Numéro du CNRCTESTOLIN1997
TESTOLIN1997A
Numéro NPARC9374296
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Identificateur de l’enregistrementded15ee1-7833-4489-bf77-4068e6faa02b
Enregistrement créé2009-07-10
Enregistrement modifié2020-03-20
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