Analysis of paralytic shellfish poisoning toxins by automated pre-column oxidation and microcolumn liquid chromatography with fluorescence detection

DOI	Trouver le DOI : https://doi.org/10.1016/0021-9673(93)80715-K
Auteur	Rechercher : Janeček, Mojmir¹; Rechercher : Quilliam, Michael A.¹; Rechercher : Lawrence, James F.
Affiliation du nom	Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
Format	Texte, Article
Résumé	Periodate oxidation of the toxins responsible for paralytic shellfish poisoning (PSP) yields fluorescent purines suitable for trace analysis by reversed-phase LC. Mobile phases containing perfluorinated acids, such as heptafluorobutyric acid, as ion-pair agents were found to provide high capacity factors for the oxidized products. Gradient elution on a microbore column with large volume injections and fluorescence detection permitted the detection of femtomole quantities of PSP toxins. A fully automated pre-column oxidation procedure was developed for an LC autosampler system in order to improve precision and allow unattended analyses. The complete method was applied successfully to various samples, including shellfish and toxic phytoplankton.
Date de publication	1993-08
Maison d’édition	ScienceDirect
Dans	Journal of Chromatography A 644, nº 2 : 321–331.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro NPARC	23000963
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	e1c0239a-6005-4338-baad-b65c716fbb10
Enregistrement créé	2016-11-22
Enregistrement modifié	2020-04-24