| Téléchargement | - Voir le manuscrit accepté : Rapid detection of microorganisms with nanoparticles and electron microscopy (PDF, 661 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1002/jemt.20614 |
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| Auteur | Rechercher : Naja, Ghinwa; Rechercher : Hrapovic, Sabahudin1; Rechercher : Male, Keith1; Rechercher : Bouvrette, Pierre1; Rechercher : Luong, John H. T.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | environmental; Escherichia coli; Rhodococcus rhodochrous; Candida sp.; electrostatic interactions |
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| Résumé | Rapid detection of microorganisms is highly desirable. A procedure has been developed based on interactions between gold nanoparticles and proteins of microorganisms (Escherichia coli, Rhodococcus rhodochrous, and Candida sp.) followed by scanning electron microscopy (SEM). The nanoparticle-cell interaction was confirmed by ultraviolet resonance Raman spectroscopy (UVRS) in the SEM focus. Cell suspensions in a buffer were interacted with gold nanoparticles (<10 nm in diameter) prepared from tetrachloroauric acid and sodium borohydride. Possible interference of elevated salt concentrations was eliminated by dialysis in deionized water. Small (10 µL) aliquots of cell-nanoparticle suspensions were dried on a silicon wafer and photographed under an SEM. Characteristic bacterial or yeast cell images in the micrographs indicated the actual presence of microorganisms in the suspension examined. This was further confirmed by UV resonance Raman spectroscopy. |
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| Date de publication | 2008-06-20 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | NRCC 49942 |
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| Numéro NPARC | 12473134 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | e7be1ee4-9ee2-4c03-9319-e6703fb218a9 |
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| Enregistrement créé | 2009-10-26 |
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| Enregistrement modifié | 2022-11-18 |
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