| DOI | Trouver le DOI : https://doi.org/10.1021/ac035396m |
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| Auteur | Rechercher : Zhao, Hui-Fen1; Rechercher : Kiyota, Taira1; Rechercher : Chowdhury, Shafinaz1; Rechercher : Purisima, Enrico1; Rechercher : Banville, Denis1; Rechercher : Konishi, Yasuo1; Rechercher : Shen, Shi-Hsiang1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | epidermal growth factor; genome; pharmaceutical; Proteins |
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| Résumé | A mammalian two-hybrid system was developed for high-throughput screening of compounds that disrupt specific protein-protein interactions. The existing mammalian systems are unsatisfactory for drug screening due to nonregulated expression of interacting proteins. To construct a tightly regulated system, the tetracycline repressor was fused with the inhibitory KRAB domain as a suppressor. The binding of the suppressor to the tet operator entirely blocked expression of two interacting proteins. When both the inducer doxycycline and drugs were added to the culture, the reporter gene was either activated by interaction of the paired proteins with ineffective drugs or remained silent due to disruption of the protein interactions by the effective drugs. We demonstrate that interactions of the type I receptor for TGFbeta with FKBP12 and the epidermal growth factor receptor (EGFR) with p85 are effectively disrupted by FK506 and EGFR kinase inhibitor AG1478, respectively. The power of this system for drug screening was further demonstrated by rapid identification of inhibitors from a druglike library for the receptor kinases |
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| Date de publication | 2004 |
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| Note | English15144206 |
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| Numéro du CNRC | 46206 |
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| Numéro NPARC | 3540257 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | e87f496d-5765-4d6a-86c5-c6ed7535a827 |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-04-17 |
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