DOI | Trouver le DOI : https://doi.org/10.1128/AEM.68.8.4035-4043.2002 |
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Auteur | Rechercher : Lanthier, Marie-Michèle1; Rechercher : Tartakovsky, Boris2; Rechercher : Villemur, R.; Rechercher : DeLuca, G.; Rechercher : Guiot, Serge2 |
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Affiliation | - Conseil national de recherches du Canada. Institut des matériaux industriels du CNRC
- Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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Format | Texte, Article |
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Résumé | Oligonucleotide probes were used to study the structure of anaerobic granular biofilm originating from a pentachlorophenol-fed upflow anaerobic sludge bed reactor augmented with Desulfitobacterium frappieri PCP-1. Fluorescence in situ hybridization demonstrated successful colonization of anaerobic granules by strain PCP-1. Scattered microcolonies of strain PCP-1 were detected on the biofilm surface after 3 weeks of reactor operation, and a dense outer layer of strain PCP-1 was observed after 9 weeks. Hybridization with probes specific for Eubacteria and Archaea probes showed that Eubacteria predominantly colonized the outer layer, while Archaea were observed in the granule interior. Mathematical simulations showed a distribution similar to that observed experimentally when using a specific growth rate of 2.2 day-1 and a low bacterial diffusion of 10-7 dm2 day-1. Also, the simulations showed that strain PCP-1 proliferation in the outer biofilm layer provided excellent protection of the biofilm from pentachlorophenol toxicity. |
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Date de publication | 2002-08-01 |
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Dans | |
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Numéro NPARC | 12339253 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | e8f40feb-20f9-41e6-9053-385bca6e3c96 |
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Enregistrement créé | 2009-09-11 |
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Enregistrement modifié | 2020-03-30 |
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