| DOI | Trouver le DOI : https://doi.org/10.1117/12.825358 |
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| Auteur | Rechercher : Tay, Li-Lin1; Rechercher : Kennedy, David1; Rechercher : Hulse, John1; Rechercher : Pezaki, John P.; Rechercher : Fraser, Jeff1; Rechercher : Wu, Xiaohua1 |
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| Éditeur | Rechercher : Kawata, Satoshi; Rechercher : Shalaev, Vladimir M.; Rechercher : Tsai, Din Ping |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences des microstructures du CNRC
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| Format | Texte, Article |
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| Conférence | SPIE NanoScience + Engineering, August 2-6, 2009, San Diego, CA, USA |
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| Sujet | plasmonics; surface enhanced Raman scattering; multimodal imaging; biosensor; nanoparticles |
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| Résumé | The plasmon resonance of noble metal nanoparticles (NP) manifests itself in a variety of extraordinary optical properties. Resonant excitation of the conduction electrons by incident radiation generates a localized surface plasmon resonance (LSPR) that is responsible for a variety of surface enhanced optical phenomena. This unique optical property coupled with well-established surface chemistry allows us to utilize both Ag and Au NP as optical contrasting agents to probe and monitor the surface receptors of cells. We have employed two plasmon-assisted optical techniques (namely, surface enhanced Raman scattering, and resonant Rayleigh scattering) to monitor the adrenergic receptors in mammalian cardiomyocyte cells that have been labeled with functionalized Ag NPs. In this study, a unique Raman reporter molecule, 4-(mercaptomethyl)benzonitrile, was developed to provide an easily identifiable vibration, the C≡N stretch, in a spectral window free from Raman bands of cell constituents and other biomolecules used in receptor crosslinking and surface passivation. Successfully labeled cells were then monitored with both optical techniques. Both techniques are related through the plasmonic properties of the noble metal NP and combined with high resolution imaging techniques; we outline the importance that different NP architectures play in the different imaging techniques. Furthermore, we will discuss the instrumentation and plasmonic implications in the design of NP best suited for such multimodal imaging approaches. |
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| Date de publication | 2009-09-02 |
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| Maison d’édition | Society of Photo-optical Instrumentation Engineers |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23004842 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | ec9cb98b-802d-4fa5-b785-0cfc2516cbd7 |
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| Enregistrement créé | 2019-01-03 |
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| Enregistrement modifié | 2020-04-16 |
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