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Cloning of a cDNA encoding the 21.2 kDa oleosin isoform from Arabidopsis thaliana and a study of its expression in a mutant defective in diacylglycerol acyltransferase activity

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/BF00021805
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Sujetoleosin; cDNA; Arabidopsis thaliana; diacylglycerol acyltransferase
Résumé
A full-length cDNA clone (pA23) of 832 bp encoding an oleosin from Arabidopsis thaliana was isolated by differential screening of a silique-specific cDNA library with probes prepared from poly(A)⁺ RNA isolated from developing seeds of wild-type (WT) Arabidopsis and from mutant AS11 with a lesion affecting diacylglycerol acyltransferase (DGAT) activity during embryo development. The encoded protein has a calculated molecular mass of 21.2 kDa, and its amino acid sequence shows strong sequence homology and structural similarity to other known oleosins. Transcription of the oleosin gene during seed development was both reduced and delayed in AS11 compared to WT. However, the level of oleosin protein did not appear to be down-regulated during seed development, and at maturity, the overall level of oleosin protein was similar in both WT and AS11. These findings indicate that regulation of oleosin gene expression is part of a highly complex, and co-ordinated expression of storage lipid biosynthesis and related (oleosin) genes during oilseed development.
Date de publication
Maison d’éditionSpringer Link
Dans
  • Plant Molecular Biology 31, nº 2 (mai 1996) : 429–433.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurBF00021805
NRCC-38925
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrementee605cd6-1efe-4b84-8bb0-a7afdd4f58aa
Enregistrement créé2023-11-02
Enregistrement modifié2023-11-02
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