| Téléchargement | - Voir la version finale : Simultaneous engineering of natural killer cells for CAR transgenesis and CRISPR-Cas9 knockout using retroviral particles (PDF, 3.2 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1016/j.omtm.2023.03.006 |
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| Auteur | Rechercher : Jo, Dong-Hyeon; Rechercher : Kaczmarek, Shelby; Rechercher : Shin, Oksu; Rechercher : Wang, Lisheng; Rechercher : Cowan, Juthaporn; Rechercher : McComb, Scott1; Rechercher : Lee, Seung-HwanIdentifiant ORCID : https://orcid.org/0000-0001-7703-6421 |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Sujet | natural killer cells; genetic engineering; CRISPR-Cas9; virus-like-particles; retroviral particles; chimeric antigen receptors; TIGIT; EGFR; flow virometry; immunotherapy |
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| Résumé | Natural killer (NK) cells are potent cytotoxic innate lymphocytes that can be used for cancer immunotherapy. Since the balance of signals from activating and inhibitory receptors determines the activity of NK cells, their anti-tumor activity can be potentiated by overexpressing activating receptors or knocking out inhibitory receptors via genome engineering, such as chimeric antigen receptor (CAR) transgenesis and CRISPR-Cas9-mediated gene editing, respectively. Here, we report the development of a one-step strategy for CRISPR-Cas9-mediated gene knockout and CAR transgenesis in NK cells using retroviral particles. We generated NK cells expressing anti-epidermal growth factor receptor (EGFR)-CAR with simultaneous TIGIT gene knockout using single transduction and evaluated the consequence of the genetic modifications in vitro and in vivo. Taken together, our results demonstrate that retroviral particle-mediated engineering provides a strategy readily applicable to simultaneous genetic modifications of NK cells for efficient immunotherapy. |
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| Date de publication | 2023-03-15 |
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| Maison d’édition | Elsevier |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | S2329050123000426 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | eec4d750-dbc7-443a-9e83-e4220c4dc60a |
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| Enregistrement créé | 2023-08-21 |
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| Enregistrement modifié | 2023-08-21 |
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