Multianalyte serum analysis using mid-infrared spectroscopy

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1177/000456329803500505
Auteur	Rechercher : Shaw, R. Anthony¹; Rechercher : Kotowich, Steven¹; Rechercher : Leroux, Michael; Rechercher : Mantsch, Henry H.¹
Affiliation	Conseil national de recherches du Canada. Institut du biodiagnostic du CNRC
Format	Texte, Article
Sujet	clinical chemistry analyses; urea; glucose; protein; albumin
Résumé	This study assesses the potential for using mid-infrared (mid-IR) spectroscopy of dried serum films as the basis for the simultaneous quantitation of eight serum analytes: Total protein, albumin, triglycerides, cholesterol, glucose, urea, creatinine and uric acid. Infrared transmission spectra were acquired for 300 serum samples, each analysed independently using accepted reference clinical chemical methods. Quantitation methods were based upon the infrared spectra and reference analyses for 200 specimens, and the models validated using the remaining 100 samples. Standard errors in the IR-predicted analyte levels (Sy/x) were 2.8 g/L (total protein), 2.2 g/L (albumin), 0.23 mmol/L (triglycerides), 0.28 mmol/L (cholesterol), 0.41 mmol/L (glucose) and 1.1 mmol/L for urea, with correlation coefficients (IR vs reference analyses) of 0.95 or better. The IR method emerged to be less suited for creatinine (Sy/x = μmol/L) and uric acid (Sy/x = 140 μmol/L) due to the relatively low concentrations typical of these analytes.
Date de publication	1998-09
Dans	Annals of Clinical Biochemistry 35, nº 5 : 624–632.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	883 NRCC 34791
Numéro NPARC	9148283
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	f619a7e2-0c77-4d17-ace3-24f0dc2106b9
Enregistrement créé	2009-06-25
Enregistrement modifié	2020-03-20

Date de modification :: 2024-07-22