| DOI | Trouver le DOI : https://doi.org/10.1002/biot.200700236 |
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| Auteur | Rechercher : Blais, David R.1; Rechercher : Alvarez-Puebla, Ramon A.2; Rechercher : Bravo-Vasquez, Juan P.2; Rechercher : Fenniri, Hicham2; Rechercher : Pezacki, John Paul1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
- Conseil national de recherches du Canada. Institut national de nanotechnologie
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| Format | Texte, Article |
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| Sujet | Antigens; barcoded resins; biothreat pathogens; multiplex immunoassays; raman spectroscopy |
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| Résumé | Suspension microsphere immunoassays are rapidly gaining recognition in antigen identification and infectious disease biodetection due to their simplicity, versatility and high-throughput multiplex screening. We demonstrate a multiplex assay based on antibody-functionalized barcoded resins (BCRs) to identify pathogen antigens in complex biological fluids. The binding event of a particular antibody on given bead (fluorescence) and the identification of the specific pathogen agent (vibrational fingerprint of the bead) can be achieved in a dispersive Raman system by exciting the sample with two different laser lines. Anthrax protective antigen, Franciscella tularensis lipopolysaccharide and CD14 antigens were accurately identified and quantified in tetraplex assays with a detection limit of 1 ng/mL. The rapid, versatile and simple analysis enabled by the BCRs demonstrates their potential for multiplex antigen detection and identification in a reconfigurable microarray format. |
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| Date de publication | 2008-06-19 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | 531
565 |
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| Numéro NPARC | 12329039 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | fc61b995-6c10-4c60-894e-65202f603161 |
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| Enregistrement créé | 2009-09-10 |
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| Enregistrement modifié | 2020-04-15 |
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