DOI | Trouver le DOI : https://doi.org/10.1002/elps.201400211 |
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Auteur | Rechercher : Guo, Tianyi; Rechercher : Wei, Yin; Rechercher : Xu, Changqing; Rechercher : Watts, Benjamin R.; Rechercher : Zhang, Zhiyi1; Rechercher : Fang, Qiyin; Rechercher : Zhang, Haiying; Rechercher : Selvaganapathy, P.Ravi; Rechercher : Deen, M. Jamal |
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Affiliation | - Conseil national de recherches du Canada. Technologies de l'information et des communications
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Format | Texte, Article |
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Sujet | polystyrene; analytical equipment; bacterial count; bacterial strain; bacterium culture; bacterium detection; biochip; concentration (parameters); flow cytometer; flow kinetics; hemocytometer; laboratory device; light scattering; microfluidics; particle size; photonic microfluidic integrated device; signal detection; suspension; Escherichia coli |
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Résumé | Counting of Escherichia coli DH5α-cell suspensions in PBS is performed using a microflow cytometer based on a photonic-microfluidic integrated device. Side-scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a hemocytometer. The detection efficiency is correlated to the ratio of sample to sheath flow rates. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2-4 μm) as their scattering intensities are different. |
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Date de publication | 2015-01-19 |
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Maison d’édition | Wiley |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 21275809 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | fcc2ed3e-a1ef-4236-a4e9-c81da5bcfdc6 |
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Enregistrement créé | 2015-07-14 |
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Enregistrement modifié | 2022-11-18 |
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