| DOI | Trouver le DOI : https://doi.org/10.1128/JB.00438-11 |
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| Auteur | Rechercher : Thomas, R.M.; Rechercher : Twine, S.M.1; Rechercher : Fulton, K.M.1; Rechercher : Tessier, L.1; Rechercher : Kilmury, S.L.N.1; Rechercher : Ding, W.1; Rechercher : Harmer, N.; Rechercher : Michell, S.L.; Rechercher : Oyston, P.C.F.; Rechercher : Titbal, R.W.; Rechercher : Prior, J.L. |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | bacterial protein; DsbA protein; hydronium ion; PilA protein; serine; threonine; unclassified drug; amino acid sequence; animal experiment; animal model; bacterial virulence; bacterium mutant; carboxy terminal sequence; female; Francisella tularensis; gene locus; liquid chromatography; mouse; nucleotide sequence; protein glycosylation; protein modification; protein structure; subcutaneous fat disorder; tandem mass spectrometry; tularemia; Bacterial Proteins; Electrophoresis, Gel, Two-Dimensional; Female; Francisella tularensis; Glycosylation; Mice; Mice, Inbred BALB C; Multigene Family; Reverse Transcriptase Polymerase Chain Reaction; Tularemia; Virulence; Virulence Factors; Francisella tularensis subsp. tularensis; Murinae |
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| Résumé | In Francisella tularensis subsp. tularensis, DsbA has been shown to be an essential virulence factor and has been observed to migrate to multiple protein spots on two-dimensional electrophoresis gels. In this work, we show that the protein is modified with a 1,156-Da glycan moiety in O-linkage. The results of mass spectrometry studies suggest that the glycan is a hexasaccharide, comprised of N-acetylhexosamines, hexoses, and an unknown monosaccharide. Disruption of two genes within the FTT0789-FTT0800 putative polysaccharide locus, including a galE homologue (FTT0791) and a putative glycosyltransferase (FTT0798), resulted in loss of glycan modification of DsbA. The F. tularensis subsp. tularensis ΔFTT0798 and ΔFTT0791::Cm mutants remained virulent in the murine model of subcutaneous tularemia. This indicates that glycosylation of DsbA does not play a major role in virulence under these conditions. This is the first report of the detailed characterization of the DsbA glycan and putative role of the FTT0789-FTT0800 gene cluster in glycan biosynthesis. © 2011, American Society for Microbiology. |
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| Date de publication | 2011 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21271529 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | ff4ea084-87c7-4727-8697-482df059dacf |
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| Enregistrement créé | 2014-03-24 |
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| Enregistrement modifié | 2020-04-21 |
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