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Fall and winter expressed sequence tags of rainbow smelt (Osmerus mordax) and the identification of two genes up-regulated during winter

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.gene.2008.08.002
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Affiliation
  1. National Research Council of Canada. Aquatic and Crop Resource Development
FormatText, Article
Abstract
The rainbow smelt (Osmerus mordax) is freeze-resistant and maintains swimming and feeding activity during winter. In order to identify genes differentially expressed in smelt liver response to winter water temperatures, a large-scale analysis of gene expression using suppression subtractive hybridization was carried out using samples obtained in fall and winter. Forward and reverse subtractions were performed, subtraction-enriched products were cloned, and clones were sequenced from both of the resulting libraries. When 27 of these genes were screened by semi-quantitative RT-PCR to identify candidates for differential expression based generally on 2-fold changes in expression, one encoding FK506-binding protein 5 was classified as up-regulated in response to seasonal change, another encoding the mitochondrial solute carrier 25 member 25 (ATP-Mg/Pi carrier) was similarly classified with seasonal change and low temperature shift, and the one encoding the 78 kDa glucose-regulated protein was provisionally classified as down-regulated with low temperature shift. Analysis of fall (warm) and winter (cold) seasonal samples by quantitative PCR (qPCR) revealed significant up-regulation of genes encoding FK506-binding protein 51 and the mitochondrial solute carrier, whereas the gene encoding the glucose-regulated protein showed no significant change in expression. The mitochondrial solute carrier and FK506-binding protein results may relate to changes in cortisol action, as both are regulated by cortisol in other species.
Publication date
In
  • Gene 424 (15 November 2008): 56–62.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC 42776
1739
NPARC number3538220
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Record identifier01cb25e4-1f83-47cd-9739-2033cb762d34
Record created2009-03-01
Record modified2020-04-15
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