Fall and winter expressed sequence tags of rainbow smelt (Osmerus mordax) and the identification of two genes up-regulated during winter

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1016/j.gene.2008.08.002
Auteur	Rechercher : Richards, Robert C.¹; Rechercher : Achenbach, John C.¹; Rechercher : Short, Connie E.; Rechercher : Kimball, Jennifer¹; Rechercher : Reith, Michael E.¹; Rechercher : Driedzic, William R.; Rechercher : Ewart, K. Vanya¹
Affiliation	Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
Format	Texte, Article
Résumé	The rainbow smelt (Osmerus mordax) is freeze-resistant and maintains swimming and feeding activity during winter. In order to identify genes differentially expressed in smelt liver response to winter water temperatures, a large-scale analysis of gene expression using suppression subtractive hybridization was carried out using samples obtained in fall and winter. Forward and reverse subtractions were performed, subtraction-enriched products were cloned, and clones were sequenced from both of the resulting libraries. When 27 of these genes were screened by semi-quantitative RT-PCR to identify candidates for differential expression based generally on 2-fold changes in expression, one encoding FK506-binding protein 5 was classified as up-regulated in response to seasonal change, another encoding the mitochondrial solute carrier 25 member 25 (ATP-Mg/Pi carrier) was similarly classified with seasonal change and low temperature shift, and the one encoding the 78 kDa glucose-regulated protein was provisionally classified as down-regulated with low temperature shift. Analysis of fall (warm) and winter (cold) seasonal samples by quantitative PCR (qPCR) revealed significant up-regulation of genes encoding FK506-binding protein 51 and the mitochondrial solute carrier, whereas the gene encoding the glucose-regulated protein showed no significant change in expression. The mitochondrial solute carrier and FK506-binding protein results may relate to changes in cortisol action, as both are regulated by cortisol in other species.
Date de publication	2008-11-15
Dans	Gene 424 (15 novembre 2008) : 56–62.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	NRCC 42776 1739
Numéro NPARC	3538220
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	01cb25e4-1f83-47cd-9739-2033cb762d34
Enregistrement créé	2009-03-01
Enregistrement modifié	2020-04-15

Date de modification :: 2024-12-26