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Somatic embryogenesis from isolated scutella of wheat: effects of physical, physiological and genetic factors

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0168-9452(96)04499-8
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectsomatic embryogenesis; Triticum aestivum; Triticum durum; genotype; cold pretreatment; physiological state
Abstract
Induction of high frequency somatic embryogenesis from in vitro culture of isolated scutella has been referred to as the Enhanced Regeneration System (ERS). In the present study, the importance of such factors as genotype, culture medium, explant size and cold pretreatment in the induction of somatic embryogenesis and regeneration response of isolated scutella was assessed in a complete block design experiment. Two species of wheat, Triticum aestivum and T. durum were compared and the findings between the two differed. The relative importance of genotype effect prevailed over all factors. In cultivars with similar embryogenic potential, the effect of explant size and cold pretreatment had major influence on the induction and regeneration frequencies. The use of an induction medium composed of amino acids and double Murashige and Skoog salt concentration, favorably increased the regeneration frequency in T. aestivum, whereas the regeneration frequency in T. durum decreased due to developmentally arrested embryos.
Publication date
PublisherElsevier
In
  • Plant Science 121, no. 1 (November 1996): 75–84.
LanguageEnglish
Peer reviewedYes
IdentifierS0168945296044998
NRCC-40021
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Record identifier06edb0ea-3fbd-41c8-8cb9-a357aaf7d3a8
Record created2023-11-02
Record modified2023-11-02
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