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Somatic embryogenesis from isolated scutella of wheat: effects of physical, physiological and genetic factors

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/S0168-9452(96)04499-8
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Sujetsomatic embryogenesis; Triticum aestivum; Triticum durum; genotype; cold pretreatment; physiological state
Résumé
Induction of high frequency somatic embryogenesis from in vitro culture of isolated scutella has been referred to as the Enhanced Regeneration System (ERS). In the present study, the importance of such factors as genotype, culture medium, explant size and cold pretreatment in the induction of somatic embryogenesis and regeneration response of isolated scutella was assessed in a complete block design experiment. Two species of wheat, Triticum aestivum and T. durum were compared and the findings between the two differed. The relative importance of genotype effect prevailed over all factors. In cultivars with similar embryogenic potential, the effect of explant size and cold pretreatment had major influence on the induction and regeneration frequencies. The use of an induction medium composed of amino acids and double Murashige and Skoog salt concentration, favorably increased the regeneration frequency in T. aestivum, whereas the regeneration frequency in T. durum decreased due to developmentally arrested embryos.
Date de publication
Maison d’éditionElsevier
Dans
  • Plant Science 121, nº 1 (novembre 1996) : 75–84.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurS0168945296044998
NRCC-40021
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Identificateur de l’enregistrement06edb0ea-3fbd-41c8-8cb9-a357aaf7d3a8
Enregistrement créé2023-11-02
Enregistrement modifié2023-11-02
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