| DOI | Resolve DOI: https://doi.org/10.1021/np050220w |
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| Author | Search for: MacKinnon, Shawna L.1; Search for: Walter, John A.1; Search for: Quilliam, Michael A.1; Search for: Cembella, Allan D.1; Search for: LeBlanc, Patricia1; Search for: Burton, Ian W.1; Search for: Hardstaff, William R.1; Search for: Lewis, Nancy I.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Marine Biosciences
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| Format | Text, Article |
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| Subject | chemistry; isolation and purification; pharmacology; imines; marine toxins; spiro compounds |
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| Abstract | Using LC/MS methodology, spirolides were detected in two clonal isolates of Alexandrium ostenfeldii isolated from Limfjorden, Denmark. Examination of the LC/MS profiles of extracts from these Danish cultures revealed the presence of two dominant peaks representing two previously unidentified spirolide components and one minor peak identified as the previously reported desmethyl spirolide C (1). Culturing of these clonal strains, LF 37 and LF 38, of A. ostenfeldii resulted in the accumulation of sufficient cell biomass to allow for the isolation and structure elucidation of two new spirolides, 13,19-didesmethylspirolide C (2) and spirolide G (3). While 2 was found to differ from 1 only in that it contained one less methyl group, 3 was the first spirolide to be isolated that contained a 5:6:6-trispiroketal ring system. The effect of this new feature on the toxicity of 3 relative to other spirolides is presently being pursued. |
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| Publication date | 2006-07-08 |
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| Terms of use | - © Copyright 2006 by the American Chemical Society and the American Society of Pharmacognosy
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 55501
1562 |
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| NPARC number | 3538091 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 09c8a761-c82b-4b2a-a991-3e7ae4602329 |
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| Record created | 2009-03-01 |
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| Record modified | 2020-04-22 |
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