| Alternative title | Application of UPLC-QTOF-MS in MSE mode for the rapid and precise identification of alkaloids in goldenseal (Hydrastis canadensis) |
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| Download | - View accepted manuscript: Separation and characterization of alkaloids in goldenseal (Hydrastis canadensis) by ultra-performance liquid chromatography coupled with electrospray ionization quadruple time of flight mass spectrometry (UPLC/Q-TOF-MS) (PDF, 1.9 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s00216-013-7558-x |
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| Author | Search for: Le, P.M.1; Search for: McCooeye, M.1; Search for: Windust, A.1 |
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| Affiliation | - National Research Council of Canada. Measurement Science and Standards
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| Format | Text, Article |
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| Abstract | Here, we describe a new application of ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MSE mode (UPLC-QTOF-MSE) for the sensitive, fast, and effective characterization of alkaloids in goldenseal (Hydrastis canadensis). This approach allowed identification of alkaloids using a cyclic low and high collision energy spectral acquisition mode providing simultaneous accurate precursor and fragment ion mass information. A total of 45 compounds were separated and 40 of them characterized including one new compound and 7 identified for the first time in goldenseal. The spectral data obtained using this method is comparable to those obtained by conventional LC-MSn. However, the UPLC-QTOF-MSE method offers high chromatographic resolution with structural characterization facilitated by accurate mass measurement in both MS and MS/MS modes in a single analytical run; this makes it suitable for the rapid analysis and screening of alkaloids in plant extracts. [Figure not available: see fulltext.] © 2014 Her Majesty the Queen in Right of Canada. |
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| Publication date | 2014 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270802 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0caece70-6f53-4350-bf02-8e7625264f7a |
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| Record created | 2014-02-17 |
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| Record modified | 2020-06-04 |
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