A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat

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DOI	Resolve DOI: https://doi.org/10.1016/j.phytochem.2020.112456
Author	Search for: Rajagopalan, Nandhakishore¹; Search for: Lu, Yuping¹; Search for: Burton, Ian W.¹; Search for: Monteil-Rivera, Fanny¹; Search for: Halasz, Annamaria²; Search for: Reimer, Elsa; Search for: Tweidt, Rebecca; Search for: Brûlé-Babel, Anita; Search for: Kutcher, Hadley R.; Search for: You, Frank M.; Search for: Cloutier, Sylvie; Search for: Cuperlovic-Culf, Miroslava³; Search for: Hiebert, Colin W.; Search for: McCallum, Brent D.; Search for: Loewen, Michele C.¹
Affiliation	National Research Council of Canada. Aquatic and Crop Resource Development National Research Council of Canada. Energy, Mining and Environment National Research Council of Canada. Digital Technologies
Format	Text, Article
Subject	Triticum aestivum L.; Poaceae; semi-targeted metabolomics; Fusarium head blight; Lr34; phenylpropanoid diglyceride
Abstract	The gene Lr34res is one of the most long-lasting sources of quantitative fungal resistance in wheat. It is shown to be effective against leaf, stem, and stripe rusts, as well as powdery mildew and spot blotch. Recent biochemical characterizations of the encoded ABC transporter have outlined a number of allocrites, including phospholipids and abscisic acid, consistent with the established general promiscuity of ABC transporters, but ultimately leaving its mechanism of rust resistance unclear. Working with flag leaves of Triticum aestivum L. variety ‘Thatcher’ (Tc) and a near-isogenic line of ‘Thatcher’ into which the Lr34res allele was introgressed (Tc+Lr34res; RL6058), a comparative semi-targeted metabolomics analysis of flavonoid-rich extracts revealed virtually identical profiles with the exception of one metabolite accumulating in Tc+Lr34res, which was not present at comparable levels in Tc. Structural characterization of the purified metabolite revealed a phenylpropanoid diglyceride structure, 1-O-p-coumaroyl-3-O-feruloylglycerol (CFG). Additional profiling of CFG across a collection of near-isogenic lines and representative Lr34 haplotypes highlighted a broad association between the presence of Lr34res and elevated accumulations of CFG. Depletion of CFG upon infection, juxtaposed to its relatively lower anti-fungal activity, suggests CFG may serve as a storage form of the more potent anti-microbial hydroxycinnamic acids that are accessed during defense responses. Altogether these findings suggest a role for the encoded LR34res ABC transporter in modifying the accumulation of CFG, leading to increased accumulation of anti-fungal metabolites, essentially priming the wheat plant for defense.
Publication date	2020-07-17
Publisher	Elsevier
In	Phytochemistry 178, 112456: 1–12.
Language	English
Peer reviewed	Yes
NRC number	NRC-ACRD-PDB046 NRCC 56461
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Record identifier	16ddd86b-8727-468e-96d2-e066ff3f9c42
Record created	2020-08-13
Record modified	2020-10-30

Date modified:: 2024-07-17