A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat

Par Conseil national de recherches du Canada

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DOI	Trouver le DOI : https://doi.org/10.1016/j.phytochem.2020.112456
Auteur	Rechercher : Rajagopalan, Nandhakishore¹; Rechercher : Lu, Yuping¹; Rechercher : Burton, Ian W.¹; Rechercher : Monteil-Rivera, Fanny¹; Rechercher : Halasz, Annamaria²; Rechercher : Reimer, Elsa; Rechercher : Tweidt, Rebecca; Rechercher : Brûlé-Babel, Anita; Rechercher : Kutcher, Hadley R.; Rechercher : You, Frank M.; Rechercher : Cloutier, Sylvie; Rechercher : Cuperlovic-Culf, Miroslava³; Rechercher : Hiebert, Colin W.; Rechercher : McCallum, Brent D.; Rechercher : Loewen, Michele C.¹
Affiliation	Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques Conseil national de recherches du Canada. Énergie, les mines et l'environnement Conseil national de recherches du Canada. Technologies numériques
Format	Texte, Article
Sujet	Triticum aestivum L.; Poaceae; semi-targeted metabolomics; Fusarium head blight; Lr34; phenylpropanoid diglyceride
Résumé	The gene Lr34res is one of the most long-lasting sources of quantitative fungal resistance in wheat. It is shown to be effective against leaf, stem, and stripe rusts, as well as powdery mildew and spot blotch. Recent biochemical characterizations of the encoded ABC transporter have outlined a number of allocrites, including phospholipids and abscisic acid, consistent with the established general promiscuity of ABC transporters, but ultimately leaving its mechanism of rust resistance unclear. Working with flag leaves of Triticum aestivum L. variety ‘Thatcher’ (Tc) and a near-isogenic line of ‘Thatcher’ into which the Lr34res allele was introgressed (Tc+Lr34res; RL6058), a comparative semi-targeted metabolomics analysis of flavonoid-rich extracts revealed virtually identical profiles with the exception of one metabolite accumulating in Tc+Lr34res, which was not present at comparable levels in Tc. Structural characterization of the purified metabolite revealed a phenylpropanoid diglyceride structure, 1-O-p-coumaroyl-3-O-feruloylglycerol (CFG). Additional profiling of CFG across a collection of near-isogenic lines and representative Lr34 haplotypes highlighted a broad association between the presence of Lr34res and elevated accumulations of CFG. Depletion of CFG upon infection, juxtaposed to its relatively lower anti-fungal activity, suggests CFG may serve as a storage form of the more potent anti-microbial hydroxycinnamic acids that are accessed during defense responses. Altogether these findings suggest a role for the encoded LR34res ABC transporter in modifying the accumulation of CFG, leading to increased accumulation of anti-fungal metabolites, essentially priming the wheat plant for defense.
Date de publication	2020-07-17
Maison d’édition	Elsevier
Dans	Phytochemistry 178, 112456 : 1–12.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	NRC-ACRD-PDB046 NRCC 56461
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	16ddd86b-8727-468e-96d2-e066ff3f9c42
Enregistrement créé	2020-08-13
Enregistrement modifié	2020-10-30

Date de modification :: 2024-06-30