| DOI | Resolve DOI: https://doi.org/10.1016/j.foodchem.2023.137341 |
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| Author | Search for: Malvestio, Cosimo; Search for: Onor, Massimo; Search for: Bramanti, Emilia; Search for: Pagliano, Enea1; Search for: Campanella, Beatrice |
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| Affiliation | - National Research Council of Canada. Metrology Research Centre
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| Format | Text, Article |
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| Subject | methionine; selenomethionine; triethyloxonioum; GC-MS; isotope dilution |
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| Abstract | A novel analytical method for the simultaneous gas chromatography–mass spectrometry (GC–MS) determination of methionine and selenomethionine in food samples is described. Samples were digested with methanesulfonic acid in a closed vessel without the need for reflux. A single step derivatization using triethyloxonium tetrafluoroborate was optimized for the conversion of the analytes into their ethyl derivatives, followed by their extraction with hexane and GC–MS analysis.. This derivatization approach was simpler and/or safer with respect to current methods based on alkyl chloroformate or silylating reagents and it yielded very clean chromatography. A design of experiment approach, based on an open source chemometric software, was used to optimize the experimental conditions. When analysis of a 1 mL volume of aqueous standard was performed, detection limits of 1 ng/g methionine and 10 ng/g for selenomethionine were obtained.
The method was validated by analysis of a selenized yeast Certified Reference Material NRC SELM-1. |
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| Publication date | 2023-08-29 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 1bbed323-0945-4b23-9a17-7ac3e9f94536 |
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| Record created | 2024-02-05 |
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| Record modified | 2025-02-13 |
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