Near-infrared fluorescence imaging of mouse myocardial microvascular endothelium using Cy5.5-lectin conjugate

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1002/jbio.201100119
Author	Search for: Nguyen, Cecilia; Search for: Bascaramurty, Saro¹; Search for: Kuzio, Bozena¹; Search for: Gregorash, Lori¹; Search for: Kupriyanov, Valery; Search for: Jilkina, Olga¹
Affiliation	National Research Council of Canada. NRC Institute for Biodiagnostics
Format	Text, Article
Subject	Cy5.5; Lycopersicon esculentum lectin; mouse; fluorescence microscopy; cardiac imaging techniques; endothelial cells
Abstract	Cy5.5-lectin, a non-toxic conjugate, combines the benefits of near-infrared (NIR) imaging, such as significant reduction of background fluorescence and increased tissue depth penetration, with its affinity for vascular endothelial cells. When compared to endothelial staining methods using FITC-lectin and ICAM2 antibodies, Cy5.5-lectin was confirmed to specifically bind endothelial cells and produce a fluorescence signal both in real-time and post-infusion. Ex-vivo experiments with isolated hearts demonstrated that binding was limited to perfused areas of the myocardium. With mouse in-vivo tail-vein injections, other organs such as the liver, spleen, and kidney were also stained and yielded similar quality images of the heart.
Publication date	2012-02-27
In	Journal of Biophotonics (27 February 2012).
Language	English
Peer reviewed	Yes
NPARC number	20243118
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Record identifier	1c95b0f7-5e26-4bcd-a613-ea22082a1b4b
Record created	2012-07-06
Record modified	2020-04-21

Date modified:: 2024-06-30