Near-infrared fluorescence imaging of mouse myocardial microvascular endothelium using Cy5.5-lectin conjugate

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1002/jbio.201100119
Auteur	Rechercher : Nguyen, Cecilia; Rechercher : Bascaramurty, Saro¹; Rechercher : Kuzio, Bozena¹; Rechercher : Gregorash, Lori¹; Rechercher : Kupriyanov, Valery; Rechercher : Jilkina, Olga¹
Affiliation	Conseil national de recherches du Canada. Institut du biodiagnostic du CNRC
Format	Texte, Article
Sujet	Cy5.5; Lycopersicon esculentum lectin; mouse; fluorescence microscopy; cardiac imaging techniques; endothelial cells
Résumé	Cy5.5-lectin, a non-toxic conjugate, combines the benefits of near-infrared (NIR) imaging, such as significant reduction of background fluorescence and increased tissue depth penetration, with its affinity for vascular endothelial cells. When compared to endothelial staining methods using FITC-lectin and ICAM2 antibodies, Cy5.5-lectin was confirmed to specifically bind endothelial cells and produce a fluorescence signal both in real-time and post-infusion. Ex-vivo experiments with isolated hearts demonstrated that binding was limited to perfused areas of the myocardium. With mouse in-vivo tail-vein injections, other organs such as the liver, spleen, and kidney were also stained and yielded similar quality images of the heart.
Date de publication	2012-02-27
Dans	Journal of Biophotonics (27 février 2012).
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro NPARC	20243118
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	1c95b0f7-5e26-4bcd-a613-ea22082a1b4b
Enregistrement créé	2012-07-06
Enregistrement modifié	2020-04-21

Date de modification :: 2024-06-30