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Solution conformation of asparagine-linked oligosaccharides: α(1-6)-linked moiety

From National Research Council Canada

Alternative titleSolution conformation of asparagine-linked oligosaccharides: alpha(1-6)-linked moiety
DOIResolve DOI: https://doi.org/10.1021/bi00284a022
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FormatText, Article
Subject1H; alpha; angle; asparagine; asparagine-linked; asparagine-linked oligosaccharides; biantennary; bond; C5-C6; calculation; carbohydrate conformation; carbohydrate sequence; chemical shift; conformation; conformational; energy; energy calculations; enhancement; glycopeptide; high-resolution; linkages; magnetic; magnetic resonance spectroscopy; mannose; models, molecular; molecule; NMR; nuclear; nuclear magnetic resonance; oligosaccharide; oligosaccharides; overhauser; overhauser enhancement; parameters; potential; resonance; shifts; solution conformation; structure
Abstract
The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of the α(1-6)-linked moiety is described. The conformational properties of these glycopeptides were determined by high-resolution 1H nuclear magnetic resonance in conjunction with potential energy calculations. The NMR parameters that were used in this analysis were chemical shifts and nuclear Overhauser enhancements. Potential energy calculations were used to evaluate the preferred conformers available for the different linkages in glycopeptides and to draw conclusions and to draw conclusions about the behavior in solution of these molecules. For all classes, identical conformations were found for the 6-arm except for the torsional angle, omega, about the C5-C6 bond of the α 1-6 linkage. For high mannose and hybrid structures omega was found to be -60 degrees, for bisected biantennary complex structures omega was 180 degrees, and for complex biantennary structures averaging between -60 degrees and 180 degrees occurs
Publication date
PublisherAmerican Chemical Society
In
  • Biochemistry 22, no. 15: 3680–3686.
LanguageEnglish
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IdentifierNRC-IBS-BRISSON1983E
NPARC number9383493
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Record identifier1dbc0b8c-85ea-4e47-ac27-3501ba756160
Record created2009-07-10
Record modified2020-03-13
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