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Solution conformation of asparagine-linked oligosaccharides: α(1-6)-linked moiety

Par Conseil national de recherches du Canada

Autre titreSolution conformation of asparagine-linked oligosaccharides: alpha(1-6)-linked moiety
DOITrouver le DOI : https://doi.org/10.1021/bi00284a022
AuteurRechercher : ; Rechercher :
FormatTexte, Article
Sujet1H; alpha; angle; asparagine; asparagine-linked; asparagine-linked oligosaccharides; biantennary; bond; C5-C6; calculation; carbohydrate conformation; carbohydrate sequence; chemical shift; conformation; conformational; energy; energy calculations; enhancement; glycopeptide; high-resolution; linkages; magnetic; magnetic resonance spectroscopy; mannose; models, molecular; molecule; NMR; nuclear; nuclear magnetic resonance; oligosaccharide; oligosaccharides; overhauser; overhauser enhancement; parameters; potential; resonance; shifts; solution conformation; structure
Résumé
The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of the α(1-6)-linked moiety is described. The conformational properties of these glycopeptides were determined by high-resolution 1H nuclear magnetic resonance in conjunction with potential energy calculations. The NMR parameters that were used in this analysis were chemical shifts and nuclear Overhauser enhancements. Potential energy calculations were used to evaluate the preferred conformers available for the different linkages in glycopeptides and to draw conclusions and to draw conclusions about the behavior in solution of these molecules. For all classes, identical conformations were found for the 6-arm except for the torsional angle, omega, about the C5-C6 bond of the α 1-6 linkage. For high mannose and hybrid structures omega was found to be -60 degrees, for bisected biantennary complex structures omega was 180 degrees, and for complex biantennary structures averaging between -60 degrees and 180 degrees occurs
Date de publication
Maison d’éditionAmerican Chemical Society
Dans
  • Biochemistry 22, nº 15 : 3680–3686.
Langueanglais
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IdentificateurNRC-IBS-BRISSON1983E
Numéro NPARC9383493
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Identificateur de l’enregistrement1dbc0b8c-85ea-4e47-ac27-3501ba756160
Enregistrement créé2009-07-10
Enregistrement modifié2020-03-13
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