| DOI | Resolve DOI: https://doi.org/10.1039/b701160a |
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| Author | Search for: Naja, Ghinwa1; Search for: Bouvrette, Pierre1; Search for: Hrapovic, Sabahudin1; Search for: Luong, John H. T.1 |
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| Affiliation | - National Research Council Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Abstract | Surface enhanced Raman scattering (SERS) has been used to detect bacteria captured by polyclonal antibodies sorbed onto protein-A-modified silver nanoparticles. The selectivity and discrimination of the technique were assured by using a specific antibody to the model bacterium, Escherichia coli. As the SERS enhancement mechanism depends upon the metal surface proximity, 8 nm was considered as the optimum distance between the bacterium and the nanoparticle surface. Spectral reproducibility was verified using Principal Components Analysis to differentiate the clusters corresponding to the biomolecules and/or bacteria sorbed onto nanoparticles. Compared to the normal Raman spectrum, the SERS technique resulted in an intensity enhancement of over 20-fold. Graphical abstract image for this article |
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| Publication date | 2007 |
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| In | |
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| Language | English |
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| NRC number | 49055 |
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| NPARC number | 3539012 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 1e039504-3b16-4cc3-99c0-1535bba6a1d9 |
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| Record created | 2009-03-01 |
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| Record modified | 2020-05-10 |
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