| DOI | Trouver le DOI : https://doi.org/10.1039/b701160a |
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| Auteur | Rechercher : Naja, Ghinwa1; Rechercher : Bouvrette, Pierre1; Rechercher : Hrapovic, Sabahudin1; Rechercher : Luong, John H. T.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Résumé | Surface enhanced Raman scattering (SERS) has been used to detect bacteria captured by polyclonal antibodies sorbed onto protein-A-modified silver nanoparticles. The selectivity and discrimination of the technique were assured by using a specific antibody to the model bacterium, Escherichia coli. As the SERS enhancement mechanism depends upon the metal surface proximity, 8 nm was considered as the optimum distance between the bacterium and the nanoparticle surface. Spectral reproducibility was verified using Principal Components Analysis to differentiate the clusters corresponding to the biomolecules and/or bacteria sorbed onto nanoparticles. Compared to the normal Raman spectrum, the SERS technique resulted in an intensity enhancement of over 20-fold. Graphical abstract image for this article |
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| Date de publication | 2007 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | 49055 |
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| Numéro NPARC | 3539012 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 1e039504-3b16-4cc3-99c0-1535bba6a1d9 |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-05-10 |
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