| DOI | Resolve DOI: https://doi.org/10.1016/S0044-328X(76)80002-5 |
|---|
| Author | Search for: Kartha, K.K.1; Search for: Gamborg, O.L.1; Search for: Shyluk, J.P.1; Search for: Constabel, F.1 |
|---|
| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
|
|---|
| Format | Text, Article |
|---|
| Subject | leaf culture; cytokinins; auxins; morphogenetic effects |
|---|
| Abstract | Leaf explants of tomato (Lycopersicon esculentum Mill. cv. Starfire) were cultured in vitro on Murashige and Skoog medium under controlled environmental conditions. Growth and morphogenetic responses of the explants to cytokinins and auxins were compared at different concentrations and combinations. Combinations of benzyladenine (BA) and indoleacetic acid (IAA) were more effective in inducing shoot differentiation than those of BA and naphthaleneacetic acid (NAA). Multiple shoot differentiation on the calli occurred at 10 μM BA with various concentrations of IAA. Rooting of the differentiated shoots was readily achieved by culturing the shoots on medium containing no growth hormones. Kinetin (K) in conjunction with IAA or NAA failed to induce shoot differentiation. Shoot and root differentiation also occurred at 0.1 μM zeatin (Z) + 10 μM IAA or 1 μM Z + 0.1 μM IAA. When used alone, BA or Z at 5 μM induced complete plant regeneration from the callus. Large numbers of regenerated plantlets were transferred to pots and grown to mature plants. |
|---|
| Publication date | 1976-02 |
|---|
| Publisher | Elsevier |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| Identifier | S0044328X76800025 |
|---|
| NRC number | NRCC 14926 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 231d7dc1-f1b2-4b40-9ebf-2c65b61156c9 |
|---|
| Record created | 2023-07-25 |
|---|
| Record modified | 2023-07-25 |
|---|
