| DOI | Trouver le DOI : https://doi.org/10.1016/S0044-328X(76)80002-5 |
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| Auteur | Rechercher : Kartha, K.K.1; Rechercher : Gamborg, O.L.1; Rechercher : Shyluk, J.P.1; Rechercher : Constabel, F.1 |
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| Affiliation | - Conseil national de recherches du Canada. Laboratoire régional des prairies
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| Format | Texte, Article |
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| Sujet | leaf culture; cytokinins; auxins; morphogenetic effects |
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| Résumé | Leaf explants of tomato (Lycopersicon esculentum Mill. cv. Starfire) were cultured in vitro on Murashige and Skoog medium under controlled environmental conditions. Growth and morphogenetic responses of the explants to cytokinins and auxins were compared at different concentrations and combinations. Combinations of benzyladenine (BA) and indoleacetic acid (IAA) were more effective in inducing shoot differentiation than those of BA and naphthaleneacetic acid (NAA). Multiple shoot differentiation on the calli occurred at 10 μM BA with various concentrations of IAA. Rooting of the differentiated shoots was readily achieved by culturing the shoots on medium containing no growth hormones. Kinetin (K) in conjunction with IAA or NAA failed to induce shoot differentiation. Shoot and root differentiation also occurred at 0.1 μM zeatin (Z) + 10 μM IAA or 1 μM Z + 0.1 μM IAA. When used alone, BA or Z at 5 μM induced complete plant regeneration from the callus. Large numbers of regenerated plantlets were transferred to pots and grown to mature plants. |
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| Date de publication | 1976-02 |
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| Maison d’édition | Elsevier |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | S0044328X76800025 |
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| Numéro du CNRC | NRCC 14926 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 231d7dc1-f1b2-4b40-9ebf-2c65b61156c9 |
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| Enregistrement créé | 2023-07-25 |
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| Enregistrement modifié | 2023-07-25 |
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