Rosette nanotubes alter IgE-Mediated degranulation in the rat basophilic leukemia (RBL)-2H3 cell line

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1093/toxsci/kfv166
Author	Search for: Ede, James D.; Search for: Ortega, Van A.; Search for: Boyle, David; Search for: Beingessner, Rachel L.¹ ; Search for: Hemraz, Usha D.¹ ; Search for: Fenniri, Hicham; Search for: Stafford, James L.; Search for: Goss, Greg G.¹
Name affiliation	NINT
Format	Text
Type	Article
Journal title	Toxicological Sciences
ISSN	1096-6080 1096-0929
Volume	148
Issue	1
Pages	108–120
Subject	degranulation; nanotoxicology; inflammation; self-assembly; rosette nanotubes
Abstract	In this study, the effects of rosette nanotube (RNT) exposure on immune cell viability and function were investigated in vitro using the rat basophilic leukemia (RBL)-2H3 cell line. RBL-2H3 viability was decreased in a dose- and time-dependent manner after lysine-functionalized RNT (K-RNT) exposure. In addition, K-RNTs had a significant effect on RBL-2H3 degranulation. When K-RNT exposure was concurrent with IgE sensitization, 50 and 100 mg l−1 K-RNTs elicited a heightened degranulatory response compared with IgE alone. Exposure to 50 and 100 mg l−1 K-RNTs also caused degranulation in RBL-2H3 cells not sensitized with IgE (0 ng ml−1 IgE). Furthermore, in cells preexposed to K-RNTs for 2 h and subsequently washed, sensitized, and stimulated with IgE, a potentiated degranulatory response was observed. Using confocal laser scanning microscopy and a fluorescein isothiocyanate (FITC)-functionalized RNT construct (termed FITC1/TBL19-RNT), we demonstrated a strong and direct affiliation between RNTs and RBL-2H3 cell membranes. We also demonstrated cellular internalization of RNTs after 2 h of exposure. Together, these data demonstrate that RNTs may affiliate with the cellular membrane of RBL-2H3 cells and can be internalized. These interactions can affect viability and alter the ability of these cells to elicit IgE-FcεR mediated degranulation.
Publication date	2015-11
Publisher	Oxford University Press
Terms of use	Permission is granted to temporarily download one copy of the materials for personal, noncommercial transitory viewing only. NRC Publications Archive - Copyright and terms of use
Language	English
Peer reviewed	Yes
NPARC number	23001601
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Record identifier	240c5e8d-ad77-49b4-b01c-deda947fd245
Record created	2017-03-09
Record modified	2017-03-09

Date modified:: 2019-03-22

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