| DOI | Resolve DOI: https://doi.org/10.1021/bc9001147 |
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| Author | Search for: Boucher, Cyril1; Search for: Liberelle, BenoÎt; Search for: Jolicoeur, Mario; Search for: Durocher, Yves1; Search for: De Crescenzo, Gregory |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | Animal; biotechnology; Biotechnology; Epidermal Growth Factor; Phosphorylation; Protein; Proteins; Tissue Engineering |
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| Abstract | We have elaborated and validated a novel approach for the oriented tethering of proteins such as the epidermal growth factor (EGF) on aminated surfaces. The grafting reactions were optimized to generate a dense and homogeneous EGF layer. Impact of EGF orientation on A-431 cellular response was investigated. Our results demonstrate that, in sharp contrast to responses obtained with soluble EGF supply or with randomly grafted EGF, oriented immobilization of EGF via a de novo designed coiled-coil capture system leads to a sustained phosphorylation of A-431 cell surface EGF receptors. Our results thus indicate that oriented protein immobilization via coiled-coil interactions is an efficient and versatile method to control tethering of bioactive molecules for future applications in the field of regenerative medicine and tissue engineering. |
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| Publication date | 2009-09-07 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 52736 |
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| NPARC number | 14021379 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 25695f56-bcf0-4d5b-ab48-f60592a19e6c |
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| Record created | 2010-06-02 |
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| Record modified | 2020-04-16 |
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