| DOI | Trouver le DOI : https://doi.org/10.1021/bc9001147 |
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| Auteur | Rechercher : Boucher, Cyril1; Rechercher : Liberelle, BenoÎt; Rechercher : Jolicoeur, Mario; Rechercher : Durocher, Yves1; Rechercher : De Crescenzo, Gregory |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | Animal; biotechnology; Biotechnology; Epidermal Growth Factor; Phosphorylation; Protein; Proteins; Tissue Engineering |
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| Résumé | We have elaborated and validated a novel approach for the oriented tethering of proteins such as the epidermal growth factor (EGF) on aminated surfaces. The grafting reactions were optimized to generate a dense and homogeneous EGF layer. Impact of EGF orientation on A-431 cellular response was investigated. Our results demonstrate that, in sharp contrast to responses obtained with soluble EGF supply or with randomly grafted EGF, oriented immobilization of EGF via a de novo designed coiled-coil capture system leads to a sustained phosphorylation of A-431 cell surface EGF receptors. Our results thus indicate that oriented protein immobilization via coiled-coil interactions is an efficient and versatile method to control tethering of bioactive molecules for future applications in the field of regenerative medicine and tissue engineering. |
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| Date de publication | 2009-09-07 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 52736 |
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| Numéro NPARC | 14021379 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 25695f56-bcf0-4d5b-ab48-f60592a19e6c |
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| Enregistrement créé | 2010-06-02 |
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| Enregistrement modifié | 2020-04-16 |
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