| DOI | Resolve DOI: https://doi.org/10.1038/nchembio726 |
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| Author | Search for: Ianoul, Anatoli1; Search for: Grant, Donna D.1; Search for: Rouleau, Yanouchka1; Search for: Bani-Yaghoub, Mahmud2; Search for: Johnston, Linda J.1; Search for: Pezacki, John Paul1 |
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| Affiliation | - National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
- National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | Domain; receptor; complexes; complex; surface |
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| Abstract | The contraction of cardiac myocytes is initiated by ligand binding to adrenergic receptors¹,² contained in nanoscale multiprotein complexes called signalosomes³. The composition and number of functional signalosomes within cardiac myocytes defines the molecular basis of the response to adrenergic stimuli³⁻⁶. For the first time, we demonstrated the ability of near-field scanning optical microscopy to visualize β-adrenergic receptors at the nanoscale in situ. On H9C2 cells, mouse neonatal and mouse embryonic cardiac myocytes, we showed that functional receptors are organized into multiprotein domains of ~140 nm average diameter. Colocalization experiments in primary cells at the nanometer scale showed that 15-20% of receptors were preassociated in caveolae. These nanoscale complexes were sufficient to effect changes in ligand-induced contraction rate without the requirement for substantial changes in receptor distribution in the cellular membrane. Using fluorescence intensities associated with these nanodomains, we estimated the receptor density within the observed nanometer features and established a lower limit for the number of receptors in the signalosome. |
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| Publication date | 2005 |
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| In | |
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| Language | English |
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| NRC number | IANOUL2005 |
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| NPARC number | 12333646 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 2cf26470-9dda-4389-a98f-8bda4bdea6b9 |
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| Record created | 2009-09-10 |
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| Record modified | 2020-04-07 |
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