| DOI | Trouver le DOI : https://doi.org/10.1038/nchembio726 |
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| Auteur | Rechercher : Ianoul, Anatoli1; Rechercher : Grant, Donna D.1; Rechercher : Rouleau, Yanouchka1; Rechercher : Bani-Yaghoub, Mahmud2; Rechercher : Johnston, Linda J.1; Rechercher : Pezacki, John Paul1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | Domain; receptor; complexes; complex; surface |
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| Résumé | The contraction of cardiac myocytes is initiated by ligand binding to adrenergic receptors¹,² contained in nanoscale multiprotein complexes called signalosomes³. The composition and number of functional signalosomes within cardiac myocytes defines the molecular basis of the response to adrenergic stimuli³⁻⁶. For the first time, we demonstrated the ability of near-field scanning optical microscopy to visualize β-adrenergic receptors at the nanoscale in situ. On H9C2 cells, mouse neonatal and mouse embryonic cardiac myocytes, we showed that functional receptors are organized into multiprotein domains of ~140 nm average diameter. Colocalization experiments in primary cells at the nanometer scale showed that 15-20% of receptors were preassociated in caveolae. These nanoscale complexes were sufficient to effect changes in ligand-induced contraction rate without the requirement for substantial changes in receptor distribution in the cellular membrane. Using fluorescence intensities associated with these nanodomains, we estimated the receptor density within the observed nanometer features and established a lower limit for the number of receptors in the signalosome. |
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| Date de publication | 2005 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | IANOUL2005 |
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| Numéro NPARC | 12333646 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 2cf26470-9dda-4389-a98f-8bda4bdea6b9 |
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| Enregistrement créé | 2009-09-10 |
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| Enregistrement modifié | 2020-04-07 |
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