| DOI | Resolve DOI: https://doi.org/10.1166/jbn.2018.2604 |
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| Author | Search for: Jin, Wuyang; Search for: Lin, Donghai1; Search for: Nguyen, Anh H.1; Search for: Abdelrasoul, Gaser N.; Search for: Chen, Jian1; Search for: Mar, Arthur; Search for: Qian, Feng; Search for: Fang, Qiang; Search for: Kovalchuk, Igor; Search for: Wang, Yutian; Search for: Chen, Jie1 |
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| Affiliation | - National Research Council of Canada. Nanotechnology
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| Format | Text, Article |
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| Subject | Enhanced Green Fluorescent Protein (EGFP); fluorescein isothiocyanate (FITC); magnetic nanoparticles; primary cortical neurons; transfection |
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| Abstract | The efficient cell transfection method is vital for various biomedical applications, such as the CRISPR-Cas9 technique. Current cell transfection methods, including lipofectamine, calcium phosphate co-precipitation, nucleofection, and viral infection are not equally efficient for various cells and have their disadvantages. In this study, a magnetic nanoparticle (MNP)-based method was introduced for delivering both FITC dye and a functional EGFP gene into easy-to-transfect HEK cells and difficult-to-transfect rat primary cortical neurons. The transfection efficacy could be controlled in both time-dependent and magnetic strength-dependent manner. This cell transfection method could have substantial potential for targeted drug delivery. |
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| Publication date | 2018-09-01 |
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| Publisher | American Scientific Publishers |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 38373a4d-82df-4b51-ba12-af9bb99ea5f3 |
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| Record created | 2019-05-03 |
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| Record modified | 2020-03-16 |
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