| DOI | Resolve DOI: https://doi.org/10.1093/protein/gzs014 |
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| Author | Search for: Hussack, G.1; Search for: Keklikian, A.; Search for: Alsughayyir, J.1; Search for: Hanifi-Moghaddam, P.1; Search for: Arbabi-Ghahroudi, M.1; Search for: Van Faassen, H.1; Search for: Hou, S.T.1; Search for: Sad, S.1; Search for: MacKenzie, R.1; Search for: Tanha, J.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | Antibody library; Complementarity-determining regions; non-aggregating; Phage display libraries; Single domains; Antibodies; Antigens; Scaffolds; Binders; antibody; bacterial antigen; bacterial protein; bacterial toxin; immunoglobulin light chain; single chain fragment variable antibody; toxB protein, Clostridium difficile; antibody library; antigen expression; codon; dissociation; enzyme linked immunosorbent assay; polyacrylamide gel electrophoresis; scanning electron microscopy; solubility; amino acid sequence; antibody affinity; chemistry; genetics; metabolism; molecular cloning; molecular genetics; nucleotide sequence; peptide library; protein binding; Amino Acid Sequence; Antibody Affinity; Antigens, Bacterial; Bacterial Proteins; Bacterial Toxins; Base Sequence; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Immunoglobulin Light Chains; Molecular Sequence Data; Peptide Library; Protein Binding; Single-Chain Antibodies |
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| Abstract | A synthetic human VL phage display library, created by the randomization of all complementarity-determining regions (CDRs) in a V L scaffold, was panned against three test antigens to determine the propensity of the library to yield non-aggregating binders. A total of 22 binders were isolated against the test antigens and the majority (20) were monomeric. Thus, human VL repertoires provide an efficient source of non-aggregating binders and represent an attractive alternative to human V H repertoires, which are notorious for containing high proportions of aggregating species. Moreover, the solubility of VLs, in contrast to VHs, appears much less CDR dependent. © The Author 2012. Published by Oxford University Press. |
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| Publication date | 2012 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21269535 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 3cce1590-8dbb-4117-8281-e32f3e6de1f7 |
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| Record created | 2013-12-12 |
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| Record modified | 2020-04-21 |
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