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Growth parameters, protein and DNA synthesis of an embryogenic suspension culture of white spruce (Picea glauca)

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0176-1617(11)80544-4
AuthorSearch for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
SubjectDNA; embryogenic suspension culture; protein; growth parameters; Picea glauca
Abstract
Growth parameters of a white spruce (Picea glauca) embryogenic suspension culture were characterized. Tissue fresh mass, dry mass, number of immature somatic embryos (stage 1), intracellular protein content, and total DNA accumulation showed similar growth curves over a 21-d culture period. Extracellular proteins gradually accumulated during the first 9 d, then dramatically increased. Immature embryo number showed a positive correlation to intracellular protein and DNA accumulation, correlation coefficients for these parameters were higher than for fresh and dry masses. The results from in vivo pulse-labelling with ³⁵S-methionine showed a peak for incorporation into proteins at day 7. The protein profiles of intracellular proteins were greatly different Erom those of extracellular proteins.
Publication date
PublisherElsevier
In
  • Journal of Plant Physiology 144, no. 2 (August 1994): 201–208.
LanguageEnglish
Peer reviewedYes
IdentifierS0176161711805444
NRCC-38003
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Record identifier41c048da-97e9-4898-95ab-0ff0b8bb264e
Record created2023-10-25
Record modified2023-10-25
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