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Growth parameters, protein and DNA synthesis of an embryogenic suspension culture of white spruce (Picea glauca)

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/S0176-1617(11)80544-4
AuteurRechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
SujetDNA; embryogenic suspension culture; protein; growth parameters; Picea glauca
Résumé
Growth parameters of a white spruce (Picea glauca) embryogenic suspension culture were characterized. Tissue fresh mass, dry mass, number of immature somatic embryos (stage 1), intracellular protein content, and total DNA accumulation showed similar growth curves over a 21-d culture period. Extracellular proteins gradually accumulated during the first 9 d, then dramatically increased. Immature embryo number showed a positive correlation to intracellular protein and DNA accumulation, correlation coefficients for these parameters were higher than for fresh and dry masses. The results from in vivo pulse-labelling with ³⁵S-methionine showed a peak for incorporation into proteins at day 7. The protein profiles of intracellular proteins were greatly different Erom those of extracellular proteins.
Date de publication
Maison d’éditionElsevier
Dans
  • Journal of Plant Physiology 144, nº 2 (août 1994) : 201–208.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurS0176161711805444
NRCC-38003
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement41c048da-97e9-4898-95ab-0ff0b8bb264e
Enregistrement créé2023-10-25
Enregistrement modifié2023-10-25
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