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Prostaglandin E2 modulation of gene expression in an Atlantic salmon (Salmo salar) macrophage-like cell line (SHK-1)

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.dci.2005.03.007
AuthorSearch for: 1; Search for: 2; Search for: 3
Affiliation
  1. National Research Council of Canada. NRC Genomics and Health Initiative
  2. National Research Council of Canada. Industrial Research Assistance Program
  3. National Research Council of Canada. NRC Institute for Marine Biosciences
FormatText, Article
SubjectReal-time PCR; gene expression; cytokines; IL-1b; TNFa; MHC I; MHC II; TGF?; COX-2; inflammation; macrophage-like cell line; prostaglandin E2; Atlantic salmon
Abstract
Following lipopolysaccharide (LPS)-stimulation of Atlantic salmon (Salmo salar) macrophage-like SHK-1 cells, prostaglandin E2 (PGE2) exhibited dose-dependent inhibition of the antigen presenting molecules major histocompatability class I and II and the pro-inflammatory cytokine interleukin-1b gene expression. Prostaglandin E2 was found to be stimulatory towards cyclooxygenase-2 (COX-2) expression at higher concentrations (1!10K6 and 1!10K8 M) and inhibitory at lower concentrations (1!10K10 and 1!10K12 M) after 4 h exposure. After 24 h exposure, however, LPS-induced COX-2 expression decreased and was completely inhibited by all PGE2 concentrations (1!10K6–1!10K10 M). Incubation of SHK-1 cells with LPS alone had no effect on tumour necrosis factor a (TNFa)-like gene or transforming growth factor b-like gene expression after 4 h, however, LPS and PGE2 showed a synergistic effect on TNFa-like gene expression after 24 h. This study provides evidence for the existence of a PGE2-mediated negative feedback mechanism in the control of PGs through down-regulation of COX-2, as well as for inflammatory responses by the down-regulation of both COX-2 and IL-1b. The differential regulation of immune-related genes under these conditions further demonstrates the usefulness of the SHK-1 cell line for studying aspects of salmonid immunology.
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Terms of use
  • Copyright © 2005 Elsevier Ltd All rights reserved.
In
  • Developmental & Comparative Immunology 29, no. 11 (2005): 951–963.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC 42476
1415
NPARC number3538549
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Record identifier456a15d8-3ef0-4bbb-b43d-cc6b81f6944b
Record created2009-03-01
Record modified2020-04-07
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