| DOI | Trouver le DOI : https://doi.org/10.1016/j.dci.2005.03.007 |
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| Auteur | Rechercher : Fast, M. D.1; Rechercher : Ross, N. W.2; Rechercher : Johnson, S. C.3 |
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| Affiliation | - Conseil national de recherches Canada. Initiative en génomique et en santé du CNRC
- Conseil national de recherches Canada. Programme d'aide à la recherche industrielle
- Conseil national de recherches Canada. Institut des biosciences marines du CNRC
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| Format | Texte, Article |
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| Sujet | Real-time PCR; gene expression; cytokines; IL-1b; TNFa; MHC I; MHC II; TGF?; COX-2; inflammation; macrophage-like cell line; prostaglandin E2; Atlantic salmon |
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| Résumé | Following lipopolysaccharide (LPS)-stimulation of Atlantic salmon (Salmo salar) macrophage-like SHK-1 cells, prostaglandin E2 (PGE2) exhibited dose-dependent inhibition of the antigen presenting molecules major histocompatability class I and II and the pro-inflammatory cytokine interleukin-1b gene expression. Prostaglandin E2 was found to be stimulatory towards cyclooxygenase-2 (COX-2) expression at higher concentrations (1!10K6 and 1!10K8 M) and inhibitory at lower concentrations (1!10K10 and 1!10K12 M) after 4 h exposure. After 24 h exposure, however, LPS-induced COX-2 expression decreased and was completely inhibited by all PGE2 concentrations (1!10K6–1!10K10 M). Incubation of SHK-1 cells with LPS alone had no effect on tumour necrosis factor a (TNFa)-like gene or transforming growth factor b-like gene expression after 4 h, however, LPS and PGE2 showed a synergistic effect on TNFa-like gene expression after 24 h. This study provides evidence for the existence of a PGE2-mediated negative feedback mechanism in the control of PGs through down-regulation of COX-2, as well as for inflammatory responses by the down-regulation of both COX-2 and IL-1b. The differential regulation of immune-related genes under these conditions further demonstrates the usefulness of the SHK-1 cell line for studying aspects of salmonid immunology. |
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| Date de publication | 2005 |
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| Conditions d’utilisation | - Copyright © 2005 Elsevier Ltd All rights reserved.
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 42476
1415 |
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| Numéro NPARC | 3538549 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 456a15d8-3ef0-4bbb-b43d-cc6b81f6944b |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-04-07 |
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