Carbohydrate profiling in seeds and seedlings of transgenic triticale modified in the expression of sucrose:sucrose-1-fructosyltransferase (1-SST) and sucrose:fructan-6-fructosyltransferase (6-SFT)

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1016/j.jbiosc.2012.05.008
Author	Search for: Diedhiou, Calliste; Search for: Gaudet, Denis; Search for: Liang, Yehong; Search for: Sung, Jinyue; Search for: Lu, Zhen-Xing; Search for: Eudes, François; Search for: Laroche, André
Format	Text, Article
Subject	Sucrose:sucrose-1-fructosyltransferase (1-SST); Sucrose:fructan-6-fructosyltransferase (6-SFT); Oligofructans; Transcripts; Stable transformation; Triticale
Abstract	Constructs with sucrose-sucrose 1-fructosyltransferase (1-SST) from rye and or sucrose-fructan 6-fructosyltransferase (6-SFT) from wheat were placed under the control of wheat aleurone-specific promoter and expressed in triticale using biolistic and microspore transformation. Transgenic lines expressing one or both the 1-SST and the 6-SFT accumulated 50% less starch and 10–20 times more fructan, particularly 6-kestose, in the dry seed compared to the untransformed wild-type (WT) triticale; other fructans ranged in size from DP 4 to DP 15. During germination from 1 to 4 days after imbibition (dai), fructans were rapidly metabolized and only in transgenic lines expressing both 1-SST and 6-SFT were fructan contents significantly higher than in the untransformed controls after 4 days. In situ hybridization confirmed expression of 6-SFT in the aleurone layer in imbibed seeds of transformed plants. When transgenic lines were subjected to a cold stress of 4°C for 2 days, synthesis of fructan increased compared to untransformed controls during low-temperature germination. The increase of fructan in dry seed and germinating seedling was generally associated with transcript expression levels in transformed plants but total gene expression was not necessarily correlated with the time course accumulation of fructan during germination. This is the first report of transgenic modification of cereals to achieve production of fructans in cereal seeds and during seed germination.
Publication date	2012-06-13
In	Journal of Bioscience and Bioengineering 114, no. 4 (13 June 2012): 371–378.
Language	English
Peer reviewed	Yes
NRC publication	This is a non-NRC publication "Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.
NRC number	NRCC 55444
NPARC number	21268515
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Record identifier	466439c9-e377-4acd-85f7-29fd91709591
Record created	2013-09-09
Record modified	2020-04-21

Date modified:: 2024-06-29