Carbohydrate profiling in seeds and seedlings of transgenic triticale modified in the expression of sucrose:sucrose-1-fructosyltransferase (1-SST) and sucrose:fructan-6-fructosyltransferase (6-SFT)

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1016/j.jbiosc.2012.05.008
Auteur	Rechercher : Diedhiou, Calliste; Rechercher : Gaudet, Denis; Rechercher : Liang, Yehong; Rechercher : Sung, Jinyue; Rechercher : Lu, Zhen-Xing; Rechercher : Eudes, François; Rechercher : Laroche, André
Format	Texte, Article
Sujet	Sucrose:sucrose-1-fructosyltransferase (1-SST); Sucrose:fructan-6-fructosyltransferase (6-SFT); Oligofructans; Transcripts; Stable transformation; Triticale
Résumé	Constructs with sucrose-sucrose 1-fructosyltransferase (1-SST) from rye and or sucrose-fructan 6-fructosyltransferase (6-SFT) from wheat were placed under the control of wheat aleurone-specific promoter and expressed in triticale using biolistic and microspore transformation. Transgenic lines expressing one or both the 1-SST and the 6-SFT accumulated 50% less starch and 10–20 times more fructan, particularly 6-kestose, in the dry seed compared to the untransformed wild-type (WT) triticale; other fructans ranged in size from DP 4 to DP 15. During germination from 1 to 4 days after imbibition (dai), fructans were rapidly metabolized and only in transgenic lines expressing both 1-SST and 6-SFT were fructan contents significantly higher than in the untransformed controls after 4 days. In situ hybridization confirmed expression of 6-SFT in the aleurone layer in imbibed seeds of transformed plants. When transgenic lines were subjected to a cold stress of 4°C for 2 days, synthesis of fructan increased compared to untransformed controls during low-temperature germination. The increase of fructan in dry seed and germinating seedling was generally associated with transcript expression levels in transformed plants but total gene expression was not necessarily correlated with the time course accumulation of fructan during germination. This is the first report of transgenic modification of cereals to achieve production of fructans in cereal seeds and during seed germination.
Date de publication	2012-06-13
Dans	Journal of Bioscience and Bioengineering 114, nº 4 (13 juin 2012) : 371–378.
Langue	anglais
Publications évaluées par des pairs	Oui
Publication du CNRC	Cette publication n’est pas du CNRC Ce sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC.
Numéro du CNRC	NRCC 55444
Numéro NPARC	21268515
Exporter la notice	Exporter en format RIS
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Identificateur de l’enregistrement	466439c9-e377-4acd-85f7-29fd91709591
Enregistrement créé	2013-09-09
Enregistrement modifié	2020-04-21

Date de modification :: 2024-06-25